Part:BBa_K223044:Experience

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[http://2011.igem.org/Team:UNICAMP-EMSE_Brazil Unicamp-EMSE (2011)] team device

• UNICAMP-EMSE (2011) team created the part BBa_K554010 modified from Stanford team anti-inflammatory device (iGEM 2009 - http://2009.igem.org/Team:Stanford/ProjectPage), which comprises SoxR gene (BBa_K223047) under control of a Constitutive Promoter (BBa_J23119) and SoxS promoter (BBa_K223001 – deleted part). Although this system has already been designed and used in previously iGEM competitions (iGEM 2009 - http://2009.igem.org/Team:Stanford/ProjectPage), we took advantage of the availability of synthesized sequences to design new parts (SoxR, and SoxS) that conforms the common biobrick standards in the iGEM registry. Our team has improved the parts as described in [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Project#UNICAMP-EMSE_Brazil_Parts_Design:_why_are_they_different_to_Stanford_2009_team_ones.3F Innovation].

• These newly designed parts were capable of sensing different concentrations of the inducer, resulting in an increased production of the protein under control of the SoxS promoter (in this case, GFP).

• Under the experimental conditions, no cell growth inhibition was observed and confirmed by the calculations of the specific growth rate (μ). No significant difference was found in control experiment (0 μM of Paraquat) and cell induced with 40 μM of Paraquat, both presented μ= 0,18 h-1. Stanford 2009 team showed that Paraquat concentrations between 60 μM and 80 μM can inhibit E. coli cell growth due to enhanced toxicity, but these concentrations were not included in our tests.

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